Characterization of Pathogenic Trypanosomatidae by Restriction Endonuclease Fingerprinting of Kinetoplast DNA Minicircles
نویسندگان
چکیده
منابع مشابه
Strains and clones of Trypanosoma cruzi can be characterized by pattern of restriction endonuclease products of kinetoplast DNA minicircles.
A simple method was developed for the characterization of different strains of Trypanosoma cruzi. T. cruzi stocks isolated from vectors or by hemoculture from patients with Chagas disease could be grouped in subpopulations having similar patterns of restriction endonuclease products of kinetoplast DNA minicircles. We designate such subpopulations by the term "schizodemes." Furthermore, it is sh...
متن کاملSequences of two kinetoplast DNA minicircles of Tryptanosoma brucei.
Kinetoplast DNA of Trypanosoma brucei is composed of a network of about 10,000 interlocked minicircle DNA molecules (1.0 kilobase) that are catenated with about 50 maxicircle DNA molecules (23 kilobases). Several different DNA . DNA hybridization techniques using individual minicircle DNA sequences cloned in Escherichia coli have indicated that each minicircle molecule contains about one-fourth...
متن کاملThe attachment of minicircles to kinetoplast DNA networks during replication.
Kinetoplast DNA (kDNA), the trypanosomatid mitochondrial DNA, is a network containing several thousand interlocked minicircles. During kDNA synthesis, minicircles dissociate from the network, and after replication their progeny reattach to the network periphery. Using electron microscopy autoradiography, we found that newly synthesized 3H-labeled minicircles, after short labeling periods, are c...
متن کاملGenomic organization of Trypanosoma brucei kinetoplast DNA minicircles.
The sequences of seven new Trypanosoma brucei kinetoplast DNA minicircles were obtained. A detailed comparative analysis of these sequences and those of the 18 complete kDNA minicircle sequences from T. brucei available in the database was performed. These 25 different minicircles contain 86 putative gRNA genes. The number of gRNA genes per minicircle varies from 2 to 5. In most cases, the gene...
متن کاملKinetoplast DNA minicircles: regions of extensive sequence divergence.
Previous work has shown that the kinetoplast minicircle DNA of Leishmania species exhibits species-specific sequence divergence and this observation has led to the development of a DNA probe-based diagnostic test for leishmaniasis. In the work reported here, we demonstrate that the minicircle is composed of three types of DNA sequences with differing specificities reflecting different rates of ...
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ژورنال
عنوان ژورنال: The American Journal of Tropical Medicine and Hygiene
سال: 1980
ISSN: 0002-9637,1476-1645
DOI: 10.4269/ajtmh.1980.29.1070